Environmental Energy Mass Spectrometry Lab.

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Matrix assisted laser desorption/ionization time-of-flight mass spectrometry
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Use/Example of Use

- Qualitative screening and molecular weight measurement of samples
- Protein identification and protein characterization
- Identifying compounds and structure​

Equipment Description

Linear MALDI-TOF MS(Matrix-Assisted Laser Desorptiom / Ionization)Àº ±¹³»¿¡¼­ ÃÖÃÊ·Î °³¹ß »ý»êµÈ Á¦Ç°À¸·Î ºÐÀÚ·®(Proteins, Peptides, Carbohydrates µî)ÀÌ Å« ½Ã·á¿Í ¸ÅÆ®¸¯½º°¡ È¥ÇÕµÈ °áÁ¤¿¡ ¼ø°£ÀûÀ¸·Î ·¹ÀÌÀú¸¦ Á¶»çÇÏ¿© ÀÌ¿ÂÈ­(MALDI) ½ÃŲ ÈÄ À̿µéÀ» ºñÇà½Ã°£Çü Áú·®ºÐ¼®±â¸¦ Åë°ú½ÃÄÑ Áú·® ´ë ÀüÇϺñ(m/z)¸¦ ÃøÁ¤ÇÏ¿© ºÐÀÚ·®À» Á¦°øÇÑ´Ù.

 

Model : Tinkerbell LT, ASTA
¡Ü Mass range : ~300 kDa
¡Ü Minimum detection : 0.01 fmol
¡Ü Resolution : Peptide > 4,000 (m/z 2,400 Da, ACTH), Protein > 1,000 (m/z 12,000 Da, Cytochrome C)
¡Ü Mass Accuracy : Peptide < 100 ppm (Internal), < 150 ppm (External), Protein < 250 ppm (Internal), < 300 ppm (External)
¡Ü Sensitivity : Peptide S/N > 10 with 1 fmol Glu-Fibrinopeptide B, Protein  S/N > 10 with 50 fmol Lysozyme ​