Environmental Energy Mass Spectrometry Lab.
- Qualitative screening and molecular weight measurement of samples
- Protein identification and protein characterization
- Identifying compounds and structure
Linear MALDI-TOF MS(Matrix-Assisted Laser Desorptiom / Ionization)Àº ±¹³»¿¡¼ ÃÖÃÊ·Î °³¹ß »ý»êµÈ Á¦Ç°À¸·Î ºÐÀÚ·®(Proteins, Peptides, Carbohydrates µî)ÀÌ Å« ½Ã·á¿Í ¸ÅÆ®¸¯½º°¡ È¥ÇÕµÈ °áÁ¤¿¡ ¼ø°£ÀûÀ¸·Î ·¹ÀÌÀú¸¦ Á¶»çÇÏ¿© ÀÌ¿ÂÈ(MALDI) ½ÃŲ ÈÄ À̿µéÀ» ºñÇà½Ã°£Çü Áú·®ºÐ¼®±â¸¦ Åë°ú½ÃÄÑ Áú·® ´ë ÀüÇϺñ(m/z)¸¦ ÃøÁ¤ÇÏ¿© ºÐÀÚ·®À» Á¦°øÇÑ´Ù.
Model : Tinkerbell LT, ASTA
¡Ü Mass range : ~300 kDa
¡Ü Minimum detection : 0.01 fmol
¡Ü Resolution : Peptide > 4,000 (m/z 2,400 Da, ACTH), Protein > 1,000 (m/z 12,000 Da, Cytochrome C)
¡Ü Mass Accuracy : Peptide < 100 ppm (Internal), < 150 ppm (External), Protein < 250 ppm (Internal), < 300 ppm (External)
¡Ü Sensitivity : Peptide S/N > 10 with 1 fmol Glu-Fibrinopeptide B, Protein S/N > 10 with 50 fmol Lysozyme